黔产钩藤茎、叶化学成分及抗肿瘤活性研究.

Uncaria rhynchophylla contains large amount of alkaloids and abounds in natural resources, and has the function of heat-clearing for calming liver and wind-calming for calming frightened. In order to make clear the material basis of stems and leaves of U. rhynchophlla, in this paper, the chemical constituents of stems and leaves from U. rhynchophylla and their antitumor activities were studied. The extracts were isolated and purified by using silical gel, Sephadex LH-20, semi-HPLC, and the structures of the compounds were identified by physicochemical property and spectrum analysis. The antitumor activities of chemical constituents against K562, HEL cell lines were assessed by MTT method. Seven compounds were isolated and identified from stems of U. rhynchophylla as 3, 4, 5-trimethoxyphenol(1),scutellarin(2), isohydrohydrogenine(3), dehydroramine(4), Vallesiachotamine(5), isorhynchophylline(6), rhynchophylline(7). Twelve compounds were isolated and identified from leaves of U. rhynchophylla as octadecanol(8), β-sitosterol(9), tridecanoicacid(10), 2-methyl-5,7-dihydroxy-chroone-7-O-β-D-glucopyranoside(11), oleanolic acid(12), quercetin(13), ivy aglycone(14), kaempferol(15),(6R, 9R )-9-hydroxymegastigman-4-en-3-one(16), ursolic(17), epicatechin(18), emodin methylether(19). The results of antitumor activities were as follows:(1)Compounds 3 and 5 inhibited the activities of HEL cell lines, and IC50 values were 17.96 and 73.01 μg·mL-1; Compound 5 inhibited the activities of K562 cell lines, and IC50 values were 16.45 μg·mL-1, which indicate that some compounds from stems of U. rhynchophylla have certain antitumor activities.(2)Compounds 1, 8,10 and 16 were isolated from this plant for the first time. This study shed the scientific light on reasonable utilization and sustainable development of plant resources. [ABSTRACT FROM AUTHOR]