响应面法优化当归中阿魏酸和藁本内酯提取工艺研究.

In order to optimize the optimum technological conditions for the simultaneous extraction of ferulic acid and ligustilide, the extraction process of ferulic acid and ligustilide from A. sinensis were optimized by ultrasonic assisted response surface. Taking the comprehensive score of ferulic acid and ligustilide content as the evaluation index and the ethanol concentration, extraction time and liquid-to-solid ratio were selected as the three main factors, and the contents of ferulic acid and ligustilide in A. sinensis were determined by 1H-qNMR. Single factor screening combined with Box-Behnken central combination design test was used to optimize the parameters of extraction process. The single-factor screening results showed that a high comprehensive score was obtained under the three conditions of 80% ethanol concentration, liquid-to-solid ratio was 12 mL·g-1, or 30 min extraction duration. Based on this, the Behnken center combination design test was designed and implemented. The results showed that the influence degree of each experimental factor on the comprehensive score was the ethanol concentration, the liquid-to-solid ratio was the second, and the extraction time was the least. The best optimum extraction conditions were as follows: ethanol concentration was 80.87%, liquid-to-solid ratio was 13.04 mL·g-1, extraction duration was 30.14 min. The verification test results were consistent with the predicted values. The quantitative resonance peaks of pyrazine, ferulic acid and ligustilide were δ 8.66 ppm, δ 6.37-6.35 ppm and δ 5.55-5.53 ppm, respectively, using DMSO-d6 as solvent and pyrazine as internal standard substance by 1H-qNMR. The precision, stability, repeatability and recovery of the method were good, and the limits of quantification and detection were low, which can meet the needs of practical analysis and testing. To sum up, the optimization method of response surface method was used to optimize the extraction process, the experimental results were accurate and reliable, good reproducibility, and suitable for the simultaneous extraction of ferulic acid and ligustilide in A. sinensis. The 1H-qNMR method had the advantages of simple operation, fast analysis speed and strong specificity, and could be used for the simultaneous determination of ferulic acid and ligustilide in A. sinensis [ABSTRACT FROM AUTHOR]