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连香树水提物和乙醇提取物的体外抗氧化研究.

Authors :
杨添雁
沙秀芬
魏 琴
李 群
Source :
Guihaia. Nov2018, Vol. 38 Issue 11, p1512-1521. 10p.
Publication Year :
2018

Abstract

In order to study on the main components and antioxidation of Cercidiphyllum japonicum aqueous extract and ethanol extract, the secondary metabolites of C. japonicum leaf were extracted by aqueous extraction and ethanol extraction and the main components of aqueous extract and ethanol extract of C. japonicum were determined. The antioxidant effects of aqueous extract and ethanol extract of C. japonicum were evaluated by in vitro antioxidant assays, such as the ability of scavenging hydroxyl radical (􀅰OH), the ability of scavenging DPPH free radical (DPPH􀅰), the ability of scavenging superoxide anion (O2 -􀅰) and the ability of reducing iron ion (Fe3+). The results showed that the main components of the aqueous extract and the ethanol extract of C. japonicum both contain kaempferol. In addition, the main components of the aqueous extract of C. japonicum also contained flavonoids such as alfalfa and isoquercetin, and the main components of the ethanol extract of C. japonicum also contained flavonoids such as naringin and quercetin 3-O-β-dglucoside. The aqueous extract and the ethanol extract of C. japonicum both had the ability of scavenge hydroxyl radicals, DPPH free radicals, superoxide anions and the ability of reducing iron ion. The antioxidant effects of the aqueous extract and the ethanol extract of C. japonicum increased with the increasing concentrations, which was dosedependent. Specifically, the ability of the aqueous extract and the ethanol extract of C. japonicum to scavenge superoxide anion (IC50 values were 0.092 mg􀅰mL-1 and 0.002 mg􀅰mL-1 respectively) was stronger than that of positive control Vc (IC50 value was 0.241 mg􀅰mL-1 ). Furthermore, the IC50 value of the aqueous extract and the ethanol extract of C. japonicum on iron ion reducing power was relatively small (the IC50 value of the aqueous extract was 0.014 mg􀅰mL-1ꎻ the IC50 value of the ethanol extract was 0.001 mg􀅰mL-1), which indicated that the total antioxidant activity of C. japonicum extracts were strong. It can be seen that the aqueous extract and the ethanol extract of C. japonicum both have good antioxidant effects, and it can be used as a potential source of natural antioxidants. [ABSTRACT FROM AUTHOR]

Details

Language :
Chinese
ISSN :
10003142
Volume :
38
Issue :
11
Database :
Academic Search Index
Journal :
Guihaia
Publication Type :
Academic Journal
Accession number :
151936421
Full Text :
https://doi.org/10.11931/guihaia.gxzw201710016