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Quantitative Determination of 15 Active Components in Lepidium meyenii with UHPLC-PDA and GC-MS.

Authors :
Xu, Yao-qing
Qiao, San-yang
Wang, Zi-qian
Cui, Meng-yao
Tan, Dao-peng
Feng, Hong
Mei, Xing-sha
Li, Geng
Cheng, Long
Source :
Journal of Analytical Methods in Chemistry. 9/1/2021, p1-10. 10p.
Publication Year :
2021

Abstract

In this study, a method using ultrahigh-performance liquid chromatography with photodiode array (UHPLC-PDA) was established and validated for the simultaneous quantification of 10 active components, including eight macamides and two glucosinolates, in Lepidium meyenii (maca). A gas chromatographic mass spectroscopy (GC-MS) method was used to determine the levels of three benzyl isothiocyanates and two sterols in maca. Liquid chromatographic separation was achieved on a Waters Acquity UHPLC HSS T3 column (2.1 mm × 100 mm, 1.8 μm) with gradient elution over 15 min. The mobile phase was (B) acetonitrile-(A) 10 mM aqueous ammonium phosphate, and the detection wavelength was 210 nm. The gas chromatographic separation was performed on an SH-Rxi-1 MS column, and the ionization mode was electron ionization (EI). Two methods were confirmed to have desirable precision (RSD < 1.58%), repeatability (RSD < 1.97%), stability (RSD < 1.76%), and good linearity (R2 ≥ 0.999) within the test range. The recoveries were in the range of 96.79–109.99%, with an RSD below 2.39%. We applied the established methods and successfully analyzed 15 compounds in maca processed under different drying conditions, providing a comprehensive reference for maca processing method of development. In summary, this study provided two rapid and effective methods for the quantification of 15 active components, which contributed to the in-depth maca quality control and provided a reference for the development of maca products. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20908865
Database :
Academic Search Index
Journal :
Journal of Analytical Methods in Chemistry
Publication Type :
Academic Journal
Accession number :
152203697
Full Text :
https://doi.org/10.1155/2021/6333989