Comparación de la cinética de remoción de la lignina de bagazos de caña de azúcar (Saccharum Officinarum) y cactácea (Opuntia Ficus-Indica), utilizando enzima lacasa.

The present study evaluates the effect of a laccase from Aspergillus sp. to expose cellulose contained in both sugarcane (Saccharum officinarum, BCA) and Opuntia-ficus cactus (BOF) bagasse. The kinetic parameters of the enzymatic activity were calculated for each of the materials, using the initial rates method at various bagasse:solution ratios (10 g:L to 50 g:L) and a total reaction time of 1 h. The assays presented a non-enzymatic removal of extractables by the buffer effect, under the evaluated reaction conditions. On the other hand, there was no cellulose or hemicellulose dissolution but there was lignin. Therefore, the results were analyzed, considering the Michaelis-Menten equation and using the disappearance rate of the glignin:L ratio as a basis. Through a Lineweaver-Burk linearization, the best fit of the values was obtained, where Vmax = 14.9566 glignin:L/h and km = 40.2011 glignin:L for BCA assays (R = 0.9933) and Vmax = 26.8300 glignin:L/h and km = 43.5575 glignin:L for BOF assays (R = 0.9972). Based on the above, although laccase presented a similar enzymatic affinity for both bagasse, a better catalytic efficiency is predicted towards lignin removal from BOF (0.6160). [ABSTRACT FROM AUTHOR]