氢吗啡酮复合丙泊酚对大鼠结直肠扩张模型的麻醉效果及机制.

Objective: To study the anesthetic effect of hydrocodone combined with propofol for painless colonoscopy and to investigate the mechanism of the analgesic effect produced by hydrocodone. Methods: Clean grade male SD rats were randomly divided into four groups: normal group; propofol group; fentanyl combined with propofol group; hydromorphone combined with propofol group. After the model was anesthetized for 10 minutes, colorectal dilation was performed using a fully lubricated balloon for once per 5 min, each lasting 30 s. The abdominal wall withdrawal reflex (AWR3) scores of each group were recorded, and epinephrine and norepinephrine content were detected by ELISA. MAPK p38 expression changes were detected by western blot. Results: (1) 10 minutes after abdominal administration of propofol, the AWR3 scores of the propofol group were significantly higher than those of the fentanyl combined with propofol group and the hydromorphone combined with propofol group (P < 0.000 1, P < 0.000 1. The lowest AWR3 scores were reached 15 minutes after abdominal administration in all three groups. 20 minutes after abdominal administration, the AWR3 scores in the fentanyl combined with propofol group and the hydromorphone combined with propofol group were higher than those in the 10 and 15 minutes, but remained low. (2) The contents of epinephrine and norepinephrine in the fentanyl combined with propofol group and the hydromorphone combined with propofol group decreased significantly compared with those in the propofol group (epinephrine: P = 0.002 3, P = 0.000 2; norepinephrine：P < 0.000 1, P < 0.000 1. (3) The p-p38/p38 ratio was significantly reduced in the fentanyl combined with propofol group and the hydromorphone combined with propofol group (P < 0.000 1, P < 0.000 1) compared with the propofol group rats. Conclusion: Hydromorphone combined with propofol can effectively inhibit the phosphorylation of p38MAPK, thereby relieving pain. [ABSTRACT FROM AUTHOR]