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Implication of long non-coding RNA NEAT1 in the pathogenesis of bacterial meningitis-induced blood-brain barrier damage.
- Source :
-
Microvascular Research . Nov2021, Vol. 138, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
-
Abstract
- Blood-brain barrier (BBB) damage is closely related to various neurological disorders, including bacterial meningitis (BM). Determining a reliable strategy to prevent BBB damage in the context of infection would be highly desirable. In the present study, we investigated the implications of the long non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) in moderating BBB damage. In vitro BBB models were developed by co-culturing hCMEC/D3 cells with glioma cells, whereupon the glioma-exposed endothelial cells (GECs) were treated with a series of mimics, inhibitors, overexpression plasmids, and shRNAs for evaluating whether NEAT1, microRNA-135a (miR-135a) and hypoxia-inducible factor 1α (HIF1α) mediated BBB integrity and permeability. Furthermore, the in vivo biological function of NEAT1 was validated in a mouse model of BBB damage. NEAT1 and HIF1α were determined to be up-regulated, while miR-135a was under-expressed in GECs. As demonstrated by chromatin immunoprecipitation and dual-luciferase reporter assays, NEAT1 could bind to miR-135a, and HIF1α was confirmed as a target of miR-135a. Either overexpression of NEAT1 or depletion of miR-135a impaired the integrity and augmented the permeability of BBB. However, HIF1α silencing could reverse the BBB damage induced by NEAT1 overexpression or by inhibition of miR-135a. In vivo experiments substantiated that knockdown of NEAT1 could alleviate BBB damage in living mice. Hence, NEAT1 knockdown prevents BBB disruption and exerts promise as a potential target for BM treatment. • The study explores the role of NEAT1/miR-135a/HIF1α axis in BBB permeability in BM. • NEAT1 and HIF1α are up-regulated while miR-135a is reduced in the GEC model. • NEAT1 can bind to miR-135a which targets HIF1α. • NEAT1 increases BBB permeability via impairing miR-135a-mediated HIF1α expression. • The study suggests molecular mechanisms of BBB damage and therapeutic targets for BM. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00262862
- Volume :
- 138
- Database :
- Academic Search Index
- Journal :
- Microvascular Research
- Publication Type :
- Academic Journal
- Accession number :
- 152393782
- Full Text :
- https://doi.org/10.1016/j.mvr.2021.104225