Multi‐walled Carbon Nanotubes and Gold Nanorod Decorated Biosensor for Detection of microRNA‐126.

In this article, we introduced a novel electrochemical biosensor for the detection of microRNA‐126. The biosensor utilizes a hybridization assay combined with multi‐walled carbon nanotubes and gold nanorod‐decorated screen‐printed carbon electrodes. For electrode preparation, gold nanorods were first immobilized onto the surface of bare and multi‐walled carbon nanotube‐modified screen‐printed carbon electrodes, and the thiol tagged‐capture probe was immobilized on the electrode surface through gold and thiol group interaction. After the immobilization, thiol tagged‐capture probe hybridized with the target sequence. Under optimum conditions, we determined limit of detection (LOD) and limit of quantification (LOQ) as high as 11 nM and 36 nM, respectively. [ABSTRACT FROM AUTHOR]