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Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells.

Authors :
Lee, Po-Yen
Lai, Yu-Hung
Liu, Po-Len
Liu, Ching-Chih
Su, Chia-Cheng
Chiu, Fang-Yen
Cheng, Wei-Chung
Hsu, Shiuh-Liang
Cheng, Kai-Chun
Chiu, Li-Yi
Kao, Tzu-En
Lin, Chia-Ching
Chang, Yo-Chen
Wang, Shu-Chi
Li, Chia-Yang
Source :
Scientific Reports. 9/16/2021, Vol. 11 Issue 1, p1-11. 11p.
Publication Year :
2021

Abstract

Amantadine hydrochloride (HCl) is commonly prescribed for treating influenza A virus infection and Parkinson's disease. Recently, several studies have indicated that the use of amantadine HCl is associated with corneal edema; however, the cytotoxic effect of amantadine HCl has not been investigated. In the present study, the effects of amantadine HCl on cell growth, proliferation, and apoptosis in bovine cornea endothelial cells, and in vitro endothelial permeability were examined. Results showed that lower doses of amantadine HCl do not affect cell growth (≤ 20 μΜ), whereas higher doses of amantadine HCl inhibits cell growth (≥ 50 μΜ), induces apoptosis (2000 μΜ), increases sub-G1 phase growth arrest (2000 μΜ), causes DNA damage (≥ 1000 μΜ), and induces endothelial hyperpermeability (≥ 1000 μΜ) in bovine cornea endothelial cells; additionally, we also found that amantadine HCl attenuates the proliferation (≥ 200 μΜ) and arrests cell cycle at G1 phase (≥ 200 μΜ) in bovine cornea endothelial cells. In the present study, we measured the cytotoxic doses of amantadine HCl on cornea endothelial cells, which might be applied in evaluating the association of corneal edema. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20452322
Volume :
11
Issue :
1
Database :
Academic Search Index
Journal :
Scientific Reports
Publication Type :
Academic Journal
Accession number :
152503351
Full Text :
https://doi.org/10.1038/s41598-021-98005-9