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Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosome.
- Source :
-
Journal of Cell Biology . Feb2021, Vol. 220 Issue 2, Following p1-14. 18p. - Publication Year :
- 2021
-
Abstract
- Centrosomes are composed of a centriolar core surrounded by a pericentriolar material (PCM) matrix that docks microtubulenucleating γ-tubulin complexes. During mitotic entry, the PCM matrix increases in size and nucleating capacity in a process called centrosome maturation. Polo-like kinase 1 (PLK1) is recruited to centrosomes and phosphorylates PCM matrix proteins to drive their self-assembly, which leads to PCM expansion. Here, we show that in addition to controlling PCM expansion, PLK1 independently controls the generation of binding sites for γ-tubulin complexes on the PCM matrix. Selectively preventing the generation of PLK1-dependent γ-tubulin docking sites led to spindle defects and impaired chromosome segregation without affecting PCM expansion, highlighting the importance of phospho-regulated centrosomal γ-tubulin docking sites in spindle assembly. Inhibiting both γ-tubulin docking and PCM expansion by mutating substrate target sites recapitulated the effects of loss of centrosomal PLK1 on the ability of centrosomes to catalyze spindle assembly. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00219525
- Volume :
- 220
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Journal of Cell Biology
- Publication Type :
- Academic Journal
- Accession number :
- 152505224
- Full Text :
- https://doi.org/10.1083/jcb.202009083