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The ameliorative effect of ellagic acid on di-(2-ethylhexyl) phthalate-induced testicular structural alterations, oxidative stress, inflammation and sperm damages in adult mice.
- Source :
-
Reproductive Biology & Endocrinology . 9/18/2021, Vol. 19 Issue 1, p1-11. 11p. - Publication Year :
- 2021
-
Abstract
- Background: Phthalates such as di (2-ethylhexyl) phthalate (DEHP) are well known exogenous substances, disrupting reproductive system function and structure. The current research demonstrated the effect of ellagic acid (EA) on DEHP-induced testicular injury in mice. Methods: Thirty-five healthy adult male mice were randomly divided to five groups; normal saline receiving group, DEHP (2 g/kg/day, dissolved in corn oil, p.o.) receiving group, DEHP (2 g/kg/day, dissolved in corn oil, p.o.) and EA receiving groups (25, 50 and 100 mg/kg/day, p.o.). Treatment duration of animals was 14 days. Body and testes weights and sperm characteristics and histological changes of testes were evaluated. Serum testosterone, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels were analyzed. In the testicular tissue, oxidative/nitrosative stress markers and inflammatory cytokine levels were measured. Results: Ellagic acid significantly reduced DEHP-induced reduction of body and testes weights. The DEHP-induced reduction of spermatogonia, primary spermatocyte and sertoli cells numbers as well as reduction of sperm vitality and progressive motility were reversed by EA. Furthermore, EA inhibited DEHP-induced alterations in serum hormone levels. These effects were associated with the reduction of DEHP-induced increased level of oxidative stress and inflammatory responses. Conclusions: Ellagic acid considerably inhibits testicular toxicity of DEHP through reducing oxidative/nitrosative stress and inflammatory responses. Our data suggest that EA may be considered as a promising agent to inhibit male reproductive toxicity induced by endocrine disrupting chemicals such as DEHP. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ELLAGIC acid
*OXIDATIVE stress
*ADULTS
*ENDOCRINE disruptors
*SERTOLI cells
Subjects
Details
- Language :
- English
- ISSN :
- 14777827
- Volume :
- 19
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Reproductive Biology & Endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 152519431
- Full Text :
- https://doi.org/10.1186/s12958-021-00830-0