High performance thin layer chromatography-densitometry method based on picosecond laser-induced fluorescence for the analysis of thioridazine and its photoproducts.

• High performance thin layer chromatography by picosecond laser induced fluorescence. • Densitometry method based on steady-state and time-resolved fluorescence detection. • Validation of the developed method and application on thioridazine photoproducts. • Laser-induced fluorescence chromatograms. • Fluorescence spectra and fluorescence lifetime of photoproducts. A densitometry method based on steady-state and time-resolved fluorescence assessments for thioridazine and its photoproducts applied on HPTLC plates has been developed. The excitation source was a picosecond diode laser emitting at 375 nm. This method was used for the analysis of the photoproducts resulted from thioridazine irradiation with 266 nm nanosecond-pulsed laser. The validation of the developed method was performed for thioridazine in terms of linearity, precision, limits of detection and quantification. Furthermore, analysis of the photoproducts of irradiated thioridazine was performed by steady-state and time-resolved fluorescence. The fluorescence spectra and fluorescence lifetime of each photoproduct were obtained and the horizontal chromatograms of fluorescence maxima were generated. [ABSTRACT FROM AUTHOR]