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Comparative metabolomics analysis of bronchial epithelium during barrier establishment after allergen exposure.
- Source :
-
Clinical & Translational Allergy . Sep2021, Vol. 11 Issue 7, p1-10. 10p. - Publication Year :
- 2021
-
Abstract
- Background: Several studies have shown a correlation between an altered metabolome and respiratory allergies. The epithelial barrier hypothesis proposes that an epithelial barrier dysfunction can result in allergic diseases development. Der p 1 allergen from house dust mite is a renowned epithelial barrier disruptor and allergy initiator due to its cysteine-protease activity. Here, we compared the metabolic profile of the bronchial epithelium exposed or not to Der p 1 during barrier establishment to understand its active role in allergy development. Methods: Calu-3 cells were cultivated in air-liquid interface cultures and exposed to either Der p 1 or Ole e 1 allergens during barrier establishment. The comparative metabolomics analysis of apical and basolateral media were performed using liquid chromatography and capillary electrophoresis both coupled to mass spectrometry. Results: We showed that epithelial barrier disruption by Der p 1 was associated with a specific metabolic profile, which was highly dependent on the state of the epithelium at the time of contact. Moreover, an apical-basolateral distribution of the metabolites was also observed, indicating a compartmentalization of the response with differential metabolic patterns. A number of metabolites were changed by Der p 1, mainly related to amino acids metabolism, such as L-arginine, L-kynurenine and L-methionine. Conclusion: This work is the first report on the metabolic response in human bronchial epithelial cells associated with cysteine-protease Der p 1 activity, which could contribute to allergy development. Moreover, it supports a reformulated epithelial barrier hypothesis that might help to explain allergies and their increasing prevalence. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20457022
- Volume :
- 11
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Clinical & Translational Allergy
- Publication Type :
- Academic Journal
- Accession number :
- 152639635
- Full Text :
- https://doi.org/10.1002/clt2.12051