Recent Advances in Biocatalysis and Metabolic Engineering.

Biocatalysis refers to the utilization of enzymes, either in purified form, or existed as part of crude cell lysate or intact cells, to catalyze single- or multi-step chemical reactions, converting synthetic molecules or natural metabolites into high-value products. Immobilization of enzymes shows superior effects on, for example, enzyme stability in reaction, enzyme reusability, and bioproducts separation [[14]]. The advantages of using of MOFs to entrap enzymes within their micropores, including minimal enzyme leaching, high enzyme loading, and activity preservation, are exploited by one-pot synthesis of the biocatalysts. Therefore, the information on the preparation of these composites using mild synthesis conditions, as well as their performance in trapping active enzymes are crucial for one-step enzyme immobilization applications using MOFs. [Extracted from the article]