干扰 CASC9 表达对结直肠癌细胞增殖、凋亡的 影响及其与 miR-195-5p/CCND1 轴的关系.

Objective To investigate the effects of long non-coding RNA cancer susceptibility candidate 9 (CASC9) on the proliferation and apoptosis of colorectal cancer cells, and to explore the relationship between its mechanism and miR-195-5p cyclin D1 (CCND1) axis. Methods The expression levels of CASC9 in the normal colon epithelial cell line NCM460 and colorectal cancer cell lines SW480, SW620 and HCT-116 were detected by real-time fluorescence quantitative PCR. Colorectal cancer cells with the highest expression of CASC9 were divided into the CASC9 interference group and control group. The cells in the CASC9 interference group were transfected with SHRNA-CASC9, and the control group with SH-NC. Cell proliferation of the two groups was detected by CCK-8, apoptosis rates of the two groups were detected by flow cytometry, and mRNA expression levels of miR-195-5p and CCND1 were detected by real-time quantitative PCR. The potential binding sites of CASC9 and miR-195-5p, and miR-195-5p and CCND1 were predicted by StarBase database, and were verified by double luciflucase reporter assay. Results Compared with NCM460 cells, the expression levels of CASC9 in SW480, SW620 and HCT-116 cells increased, and the expression level of CASC9 in SW480 cells was higher than those in SW620 and HCT-116 cells (all P<0. 01). SW480 cells were selected for subsequent experiments. Compared with the control group, the proliferation ability of CASC9 cells decreased (P<0. 01), the apoptosis rate increased (P< 0. 05), and the expression levels of miR-195-5p and CCND1 mRNA increased in the interference group (both P<0. 01). StarBase database analysis showed that CASC9 could target and adsorb miR-195-5p. CCND1 was the downstream target protein of miR-195-5p. Luciferase reporter gene results showed that CASC9 could specifically bind with miR-195-5p (P< 0. 01). Conclusion CASC9 is highly expressed in colorectal cancer tissues and cells, and its mechanism may be that CASC9, as a miR-195-5p adsorption sponge, regulates the expression of CCND1 and promotes the proliferation of colorectal cancer cells and thus inhibits apoptosis. [ABSTRACT FROM AUTHOR]