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Co-immunizing with HMGB1 enhances anti-tumor immunity of B7H3 vaccine in renal carcinoma.
- Source :
-
Molecular Immunology . Nov2021, Vol. 139, p184-192. 9p. - Publication Year :
- 2021
-
Abstract
- • H1-pHMGB1/pB7H3 vaccine enhanced the induction and mature of DCs. • H1-pHMGB1/pB7H3 could induce tumor-specific CD8+ T cell responses. • H1-pHMGB1/pB7H3 vaccine could inhibit the tumor growth of renal cancer. • CD8+ T cells are required for the induction of protective anti-tumor immunity by H1-pHMGB1/pB7H3. Metastatic renal carcinoma is a kind of tumor with high degree of malignancy, but there are no effective treatment methods and strategies at present. In this study, we designed a folate-grafted PEI600-CyD (H1) nanoparticle-mediated DNA vaccine containing an adjuvant of high mobility group box 1 protein (HMGB1) and a tumor-specific antigen of B7H3 (CD276) for renal carcinoma therapy. Mice bearing subcutaneous human B7H3 (hB7H3)-Renca tumor were immunized with H1-pHMGB1/pB7H3, H1-pB7H3, H1-pHMGB1, or Mock vaccine. Compared to other control groups, the growth of the tumor was significantly inhibited in H1-pHMGB1/pB7H3 vaccine group. The increased proportion and mature of CD11c+ DCs were observed in the spleen of H1-pHMGB1/pB7H3 treated mice. Likewise, HMGB1 promoted B7H3 vaccine to induce tumor-specific CD8+ T cell proliferation and CTL responses. Beyond that, H1-pHMGB1/pB7H3 vaccine strengthened the induction of functional CD8+ T cells. With the depletion of CD8+ T cells, the anti-tumor effect of H1-pHMGB1/pB7H3 also disappeared, indicating that CD8+ T cells are the key factor of the anti-tumor activity of the vaccine. So, to sum up, H1-pHMGB1/pB7H3 vaccine could achieve the desired anti-tumor effect by enhancing the response of tumor-specific functional CD8+ T cell responses. H1 nanoparticle-based vaccines may have great potential and prospect in the treatment of primary solid tumors. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CYTOTOXIC T cells
*HIGH mobility group proteins
*DNA vaccines
Subjects
Details
- Language :
- English
- ISSN :
- 01615890
- Volume :
- 139
- Database :
- Academic Search Index
- Journal :
- Molecular Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 152768755
- Full Text :
- https://doi.org/10.1016/j.molimm.2021.09.002