Back to Search
Start Over
MiR-BART2-3p targets Unc-51-like kinase 1 and inhibits cell autophagy and migration in Epstein-Barr virus-associated gastric cancer.
- Source :
-
Virus Research . Nov2021, Vol. 305, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
-
Abstract
- • The protein levels of ULK1 were down-regulated in EBV-associated gastric carcinoma cell lines compared with EBV-negative gastric carcinoma. • EBV-miR-BART2-3p targets the 3'UTR of ULK1 and regulates its expression. • miR-BART2-3p regulates autophagy and migration by targeting ULK1, but does not effect cell proliferation. ULK1 (Unc-51-like kinase 1) is an evolutionarily conserved serine/threonine kinase that plays a central role in the regulation of autophagy. ULK1 is associated with prognosis for metastasis and survival in several tumors. However, its relationship with Epstein-Barr virus (EBV) has not been studied. We found that the expression of ULK1 in EBV-associated gastric cancer cells was lower than that in EBV-negative gastric cancer cells. Further, a luciferase reporter gene assay showed that miR-BART2-3p directly targets ULK1. EBV-miR-BART2-3p attenuated endogenous protein expression levels of some autophagy-related genes. MiR-BART2-3p could thus be involved in the regulation of autophagy. Most important, our research indicates that miR-BART2-3p targets ULK1, resulting in downregulation of epithelial-mesenchymal transformation (EMT) -associated marker proteins and reducing EMT and cell migration. Our study shows that modulation of ULK1 is the likely mechanism by which miR-BART2-3p participates in the regulation of autophagy and cancer cell migration. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01681702
- Volume :
- 305
- Database :
- Academic Search Index
- Journal :
- Virus Research
- Publication Type :
- Academic Journal
- Accession number :
- 152814881
- Full Text :
- https://doi.org/10.1016/j.virusres.2021.198567