Impact of Bioreactor Geometry on Mesenchymal Stem Cell Production in Stirred‐Tank Bioreactors.

Human mesenchymal stem cells (hMSCs) are manufactured as cell therapy products. This requires a tightly controlled and fully understood production process, usually taking place in a stirred‐tank bioreactor. In this review, the impact of bioreactor geometry as well as equipment‐related parameters is discussed. We provide a theoretical background for the engineering of hMSC spheroids with a defined size and evaluate the process parameters needed for the microcarrier‐based expansion of hMSCs. Finally, and based on experimental data, ideal bioreactor setups for each type of process are recommended. [ABSTRACT FROM AUTHOR]