Self-assembled polymeric micelle as a novel mRNA delivery carrier.

mRNA-based therapy has been evaluated in preclinical and clinical studies for the treatment of a wide variety of disease such as cancer immunotherapies and infectious disease vaccines. However, it remains challenging to development safe and efficient delivery system. Here, we have designed a novel self-assembled polymeric micelle based on vitamin E succinate modified polyethyleneimine copolymer (PVES) to delivery mRNA. In vitro , PVES could transfect mRNA into multiple cell lines such as HEK-293T, HeLa and Vero and the transfection efficiencies were much higher than PEI 25 k. In addition, the cytotoxicity of PVES was much lower than PEI 25 k. Furthermore, mice administered intramuscularly with PVES/SARS-CoV-2 mRNA vaccine induced potent antibody response and show no obvious toxicity. These results demonstrated the potential of PVES as a safe and effective delivery carrier for mRNA. [Display omitted] • Development of self-assembled polymeric micelle (PVES) as a novel delivery carrier for mRNA vaccine. • PVES transfect mRNA into multiple cell lines in vitro and the cytotoxicity is much lower. • PVES deliver SARS-CoV-2 RBD mRNA vaccine in vivo and induce potent humoral and cellular immune responses and show no obvious toxicity. • PVES is a safe and effective delivery vector for mRNA vaccine. [ABSTRACT FROM AUTHOR]