Back to Search
Start Over
Discovery and characterization of a novel α-l-fucosidase from the marine-derived Flavobacterium algicola and its application in 2′-fucosyllactose production.
- Source :
-
Food Chemistry . Feb2022, Vol. 369, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- [Display omitted] • A novel marine-derived α- l -fucosidase OUC-Jdch16 was cloned and characterized. • The OUC-Jdch16 was applied to produce 2′-FL with the highest yield of 92.15%. • Other novel glycosides were prepared via the OUC-Jdch16. 2′-Fucosyllactose (2′-FL) is one of the nutrient ingredients in human milk, which has various beneficial health effects. α- l -fucosidase is a biotechnological tool for 2′-FL preparation. Here, a novel and efficient α- l -fucosidase OUC-Jdch16 from the fucoidan-digesting strain Flavobacterium algicola 12076 was heterologously expressed and applied to produce 2′-FL in vitro. OUC-Jdch16 belongs to glycoside hydrolases (GH) family 29 and exhibits the highest 4-nitrophenyl-α- l -fucopyranoside-hydrolyzing activity at 25 °C and pH 6.0. OUC-Jdch16 could catalyze the synthesis of 2′-FL via transferring the fucosyl residue from pNP-α-fucose to lactose. Under the optimal transfucosylation conditions, the yield of the transfucosylation product reached 84.82% and 92.15% (mol/mol) from pNP-α-fucose within 48 h and 120 h, respectively. Moreover, OUC-Jdch16 was capable of transferring the fucosyl residue to other glycosyl receptors with the generation of novel fucosylated compounds. This study demonstrated that OUC-Jdch16 could be a promising tool to prepare 2′-FL and other novel glycosides. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FLAVOBACTERIUM
*GLYCOSIDASES
*BREAST milk
Subjects
Details
- Language :
- English
- ISSN :
- 03088146
- Volume :
- 369
- Database :
- Academic Search Index
- Journal :
- Food Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 153034990
- Full Text :
- https://doi.org/10.1016/j.foodchem.2021.130942