Ratlarda Rottlerin'in Otofaji Üzerine Etkili Doz Düzeyinin Araştırılması.

Objective: Autophagy is a physiological process playing role in cellular homeostasis. However, autophagic anomalies are associated with many pathological conditions such as cancer, neurodegenerative diseases, and cardiovascular diseases. Rottlerin is an important autophagic activator, which may be used to evaluate the relationship of autophagy with diseases and may be applied in treatment strategies. Nevertheless, there are no studies in the literature regarding the doses of this molecule in activating autophagy in rats. The aim of this study is to investigate the effective dose level of Rottlerin on autophagy in rats. Method: Twenty four rats were divided into 4 groups with 6 rats each. The solvent (DMSO) of the Rottlerin was given to Group I (control group). Group II, III, and IV were administered Rottlerin(0.5, 2, and 4 mg/kg) intraperitoneally, respectively. The heart tissues of rats were examined in terms of autophagic vacuole, LC3B levels, and anti-LC3B staining pattern. In addition, LC3B levels were measured in blood samples. Results: There was no significant difference between the groups in terms of autophagic vacuole, LC3B levels, and anti-LC3B staining pattern in heart tissues. As the dose of the Rottlerin increased, a tendency to increase in serum LC3B levels was observed. However, the differences were not statistically significant. Conclusion: In conclusion, according to our findings, it is seen that the dose and/or application time should be increased in order to activate autophagy in heart tissue by using Rottlerin in rats. However, serum results indicate that autophagic activity may be increased in different tissues. [ABSTRACT FROM AUTHOR]