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Two-photon ESIPT-based fluorescent probe using 4-hydroxyisoindoline-1,3-dione for the detection of peroxynitrite.
- Source :
-
Chemical Communications . 10/28/2021, Vol. 57 Issue 84, p11084-11087. 4p. - Publication Year :
- 2021
-
Abstract
- Excited-state intramolecular proton transfer (ESIPT)-based fluorophores with two-photon excitation fluorescence (TPEF) are rare. Our aim with this research was to develop ESIPT-based fluorophores exhibiting TPEF. Herein, we used 4-hydroxyisoindoline-1,3-dione as a scaffold to develop a two-photon fluorescent probe BHID-Bpin, for the detection of peroxynitrite (ONOO−). BHID-Bpin exhibits excellent selectivity, sensitivity, and fast response towards ONOO− in PBS buffer solution (10 mM, pH = 7.40). Additionally, BHID-Bpin displays high photo-stability under two-photon irradiation at 750 nm. Furthermore, the probe can image endogenous ONOO− in HeLa cells and exogenous ONOO− in rat hippocampal slices at a depth of 110 μm. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FLUORESCENT probes
*PEROXYNITRITE
*HELA cells
*BUFFER solutions
*FLUORESCENCE
Subjects
Details
- Language :
- English
- ISSN :
- 13597345
- Volume :
- 57
- Issue :
- 84
- Database :
- Academic Search Index
- Journal :
- Chemical Communications
- Publication Type :
- Academic Journal
- Accession number :
- 153152608
- Full Text :
- https://doi.org/10.1039/d1cc03160k