miR-152 Regulates Bovine Myoblast Proliferation by Targeting KLF6.

Simple Summary: Our study found that miR-152 functions in bovine myoblasts to inhibit proliferation. Our prediction and experimental verification revealed that Kruppel-Like Factor 6 (KLF6) is a direct target gene of miR-152. Therefore, miR-152 and its target gene KLF6 have certain effects on the development of bovine skeletal muscles. Though miRNAs have been reported to regulate bovine myoblast proliferation, but many miRNAs still need to be further explored. Specifically, miR-152 is a highly expressed miRNA in cattle skeletal muscle tissues, but its function in skeletal muscle development is unknown. Herein, we aimed to investigate the role of miR-152 in regulating bovine myoblast proliferation. Functionally, RT-qPCR, Western blotting, EdU assay, and flow cytometry detection results showed that miR-152 inhibited bovine myoblast proliferation. Mechanistically, we demonstrated transcription factor KLF6 was a target gene of miR-152 by means of bioinformatics software prediction and dual-luciferase report analysis, which had been demonstrated to be favorable for myoblast proliferation. Collectively, our research suggested that miR-152 inhibits bovine myoblast proliferation via targeting KLF6. [ABSTRACT FROM AUTHOR]