Eotaxin (CCL11) enhances mediator release from human basophils.

Eotaxin at 10 nM significantly enhanced basophil histamine release triggered by anti-IgE antibody and C5a (Figure 1A); release was similarly enhanced in highly pure (purity: ~90%) basophils (Figure 1B). Keywords: allergy; basophils; eotaxin (CCL11); histamine; leukotriene C4 EN allergy basophils eotaxin (CCL11) histamine leukotriene C4 3549 3552 4 11/01/21 20211101 NES 211101 ACKNOWLEDGEMENTS The authors thank Drs Kei Onuma, Ryo Hatano (Juntendo University) and Hiroshi Kawasaki (JCHO Yugawara Hospital) for kindly providing us with anti-CCR3 hybridoma, Ms Sayaka Igarashi for helpful advice on the experimental procedures, Dr Hirokazu Sakasegawa for his critical reading of this manuscript, and Ms Yasuko Asada for her excellent secretarial work. [Extracted from the article]