Study on the Genetic Transformation Conditions of Begonia wallichiana L. with Leaf Disc Method and Corresponding Identification Techniques.

[Objectives] This study was conducted to improve the Agrobacterium-mediated genetic transformation system of Begonia wallichiana. [Methods] With sterilized tube seedling leaves as the recipient material and GBP as the reporter gene, optimization experiments were carried out in terms of infection time and method, co-cultivation time and method, and PCR detection technology. [Results] The transformation effect was better under the conditions of shaking Agrobacterium liquid, infection time of 1 -2 h, and co-cultivation on sterilized filter paper for 2 d. After co-cultivation, the recipient material was first subjected to recover}' culture, and then used for Hyg gradient screening, which was conducive to obtaining resistant transformants. The designed specific PCR detection technology could quickly identify false positives in resistant regenerated plants, and the proportion of transgenic plants was 16.7%. [Conclusions]The research results provide a new technical reference for the genetic transformation of ornamental plants. [ABSTRACT FROM AUTHOR]