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Expression of STOML2 promotes proliferation and glycolysis of multiple myeloma cells via upregulating PAI-1.
- Source :
-
Journal of Orthopaedic Surgery & Research . 11/13/2021, Vol. 16 Issue 1, p1-9. 9p. - Publication Year :
- 2021
-
Abstract
- Background: This study aimed to investigate the effects of STOML2 and the relationship between STOML2 and PAI-1 in the development of multiple myeloma (MM). Methods: Cell proliferation was tested using CCK-8 assay and cell colony formation assay. Glucose consumption, lactate production and ATP level were measured using commercial kits. The mRNA and protein expression were assessed using quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting, respectively. Results: Both mRNA and protein expression of STOML2 were upregulated in MM patients compared to healthy volunteers. CCK-8 and colony formation assays demonstrated that STOML2 silencing inhibited cell proliferation in MM cells. Knockdown of STOML2 reduced glucose consumption, lactate production and ATP/ADP ratios. STOML2 silencing by shSTOML2 led to reduced PAI-1 expression. Overexpression of PAI-1 reversed the inhibitory effects of shSTOML2 on MM cell growth. Conclusion: Results from this study demonstrated that STOML2 silencing inhibits cell proliferation and glycolysis through downregulation of PAI-1 expression, suggesting a new therapeutic target for MM. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1749799X
- Volume :
- 16
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Orthopaedic Surgery & Research
- Publication Type :
- Academic Journal
- Accession number :
- 153552950
- Full Text :
- https://doi.org/10.1186/s13018-021-02819-2