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Two isomeric and distinguishable H2S fluorescence probes for monitoring spoilage of eggs and visualizing exogenous and endogenous H2S in living cells.
- Source :
-
Analyst . 1/7/2020, Vol. 145 Issue 1, p213-222. 10p. - Publication Year :
- 2020
-
Abstract
- Accurate fabrication of fluorescence probes to efficiently monitor and detect H2S levels in the fields of foodstuffs and physiology is crucial. Herein, we report two isomeric phenanthro[9,10-d]imidazole benzene sulfonamide-derived fluorescence probes (PI-2-SA and PI-4-SA), both of which display remarkable responses toward H2S over other analytes. The spectral characteristics of the two probes were investigated and are discussed in detail. By comparison, PI-2-SA was specific, sensitive (the limit of detection was ca. 12.3 nM), rapid (within ≤3 min) and dynamic (the rate constant was 0.02 s−1). Significantly, PI-2-SA was proved to be effective in monitoring the shelf-time progress of egg samples in real time as well as in the imaging of exogenous and endogenous H2S in HeLa cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FLUORESCENCE
*HELA cells
*DETECTION limit
*IMIDAZOLES
Subjects
Details
- Language :
- English
- ISSN :
- 00032654
- Volume :
- 145
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 153628528
- Full Text :
- https://doi.org/10.1039/c9an01629e