不同 fimA 基因型牙龈卟啉单胞菌对人脐静脉内皮细胞分泌相关蛋白及 细胞因子的影响.

BACKGROUND: Porphyromonas gingivalis infection is a risk factor that has been found to be related to atherosclerosis and other diseases in recent years. However, different fimA genotypes of Porphyromonas gingivalis have different pathogenic abilities. Actively explore Porphyromonas gingivalis high-risk bacterial strains play an important role in improving the diagnosis and treatment mechanism of atherosclerosis. OBJECTIVE: To investigate the effects of different fimA genotypes of Porphyromonas gingivalis on the secretion of monocyte chemoattractant protein-1, interleukin-6, and endothelin-1/nitric oxide in human umbilical vein endothelial cells. METHODS: Porphyromonas gingivalis genotypes IV, II, and I fimA were cultured under anaerobic conditions, and the multiplicity of infection was 100 to infect human umbilical vein endothelial cells. In the positive control group, Porphyromonas gingivalis lipopolysaccharide was used to stimulate human umbilical vein endothelial cells, and in the negative control group, human umbilical vein endothelial cells were cultured with cell culture medium only. Enzyme-linked immunosorbent assay was used to detect the levels of monocyte chemoattractant protein 1, interleukin 6, endothelin 1/nitric oxide in the culture supernatant of human umbilical vein endothelial cells after 2, 6, and 24 hours in each group. RESULTS AND CONCLUSION: Except for the negative control group, the levels of interleukin 6, endothelin 1/nitric oxide, and monocyte chemoattractant protein 1 in the culture supernatant of human umbilical vein endothelial cells in the other four groups gradually increased. The levels of interleukin 6, endothelin 1/ nitric oxide in the IV fimA genotype stimulation group were higher than those in the II and I fimA genotype stimulation groups and the positive control group and the negative control group (P < 0.05). The level of monocyte chemoattractant protein 1 in the II fimA genotype stimulation group was higher than that in the IV, I fimA genotype stimulation group and the negative control group (P < 0.05). The results indicate that there are certain differences in the dysfunction mechanisms of Porphyromonas gingivalis with different fimA genotypes in stimulating human umbilical vein endothelial cells. The II fimA genotype and IV fimA gene have a strong ability to up-regulate the secretion of monocyte chemoattractant protein 1, interleukin 6, and endothelin 1/nitric oxide, and may play a dominant role in causing vascular vein endothelial cell dysfunction. [ABSTRACT FROM AUTHOR]