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Developing a mechanically matched decellularized spinal cord scaffold for the in situ matrix-based neural repair of spinal cord injury.

Authors :
Ma, Yuan-huan
Shi, Hui-juan
Wei, Qing-shuai
Deng, Qing-wen
Sun, Jia-hui
Liu, Zhou
Lai, Bi-qin
Li, Ge
Ding, Ying
Niu, Wan-ting
Zeng, Yuan-shan
Zeng, Xiang
Source :
Biomaterials. Dec2021, Vol. 279, pN.PAG-N.PAG. 1p.
Publication Year :
2021

Abstract

Tissue engineering is a promising strategy to repair spinal cord injury (SCI). However, a bioscaffold with mechanical properties that match those of the pathological spinal cord tissue and a pro-regenerative matrix that allows robust neurogenesis for overcoming post-SCI scar formation has yet to be developed. Here, we report that a mechanically enhanced decellularized spinal cord (DSC) scaffold with a thin poly (lactic-co-glycolic acid) (PLGA) outer shell may fulfill the requirements for effective in situ neuroengineering after SCI. Using chemical extraction and electrospinning methods, we successfully constructed PLGA thin shell-ensheathed DSC scaffolds (PLGA-DSC scaffolds) in a way that removed major inhibitory components while preserving the permissive matrix. The DSCs exhibited good cytocompatibility with neural stem cells (NSCs) and significantly enhanced their differentiation toward neurons in vitro. Due to the mechanical reinforcement, the implanted PLGA-DSC scaffolds showed markedly increased resilience to infiltration by myofibroblasts and the deposition of dense collagen matrix, thereby creating a neurogenic niche favorable for the targeted migration, residence and neuronal differentiation of endogenous NSCs after SCI. Furthermore, PLGA-DSC presented a mild immunogenic property but prominent ability to polarize macrophages from the M1 phenotype to the M2 phenotype, leading to significant tissue regeneration and functional restoration after SCI. Taken together, the results demonstrate that the mechanically matched PLGA-DSC scaffolds show promise for effective tissue repair after SCI. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01429612
Volume :
279
Database :
Academic Search Index
Journal :
Biomaterials
Publication Type :
Academic Journal
Accession number :
153827052
Full Text :
https://doi.org/10.1016/j.biomaterials.2021.121192