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DNA Tiling Enables Precise Acylation‐Based Labeling and Control of mRNA.
- Source :
-
Angewandte Chemie International Edition . 12/13/2021, Vol. 60 Issue 51, p26798-26805. 8p. - Publication Year :
- 2021
-
Abstract
- Methods for the site‐selective labeling of long, native RNAs are needed for studying mRNA biology and future therapies. Current approaches involve engineering RNA sequences, which may alter folding, or are limited to specific sequences or bases. Here, we describe a versatile strategy for mRNA conjugation via a novel DNA‐tiling approach. The method, TRAIL, exploits a pool of "protector" oligodeoxynucleotides to hybridize and block the mRNA, combined with an "inducer" DNA that extrudes a reactive RNA loop for acylation at a predetermined site. Using TRAIL, an azido‐acylimidazole reagent was employed for labeling and controlling RNA for multiple applications in vitro and in cells, including analysis of RNA‐binding proteins, imaging mRNA in cells, and analysis and control of translation. The TRAIL approach offers an efficient and accessible way to label and manipulate RNAs of virtually any length or origin without altering native sequence. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DNA
*RNA-binding proteins
*CELL imaging
*RNA
*PROTEIN analysis
Subjects
Details
- Language :
- English
- ISSN :
- 14337851
- Volume :
- 60
- Issue :
- 51
- Database :
- Academic Search Index
- Journal :
- Angewandte Chemie International Edition
- Publication Type :
- Academic Journal
- Accession number :
- 153983361
- Full Text :
- https://doi.org/10.1002/anie.202112106