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Correlation of Acinetobacter baumannii K144 and K86 capsular polysaccharide structures with genes at the K locus reveals the involvement of a novel multifunctional rhamnosyltransferase for structural synthesis.
- Source :
-
International Journal of Biological Macromolecules . Dec2021:Part B, Vol. 193, p1294-1300. 7p. - Publication Year :
- 2021
-
Abstract
- Whole genome sequence from Acinetobacter baumannii isolate Ab-46-1632 reveals a novel KL144 capsular polysaccharide (CPS) biosynthesis gene cluster, which carries genes for d -glucuronic acid (D -GlcA) and l -rhamnose (l -Rha) synthesis. The CPS was extracted from Ab-46-1632 and studied by 1H and 13C NMR spectroscopy, including a two-dimensional 1H,13C HMBC experiment and Smith degradation. The CPS was found to have a hexasaccharide repeat unit composed of four l -Rha p residues and one residue each of d -Glc p A and N -acetyl- d -glucosamine (D -Glc p NAc) consistent with sugar synthesis genes present in KL144. The K144 CPS structure was established and found to be related to those of A. baumannii K55, K74, K85, and K86. A comparison of the corresponding gene clusters to KL144 revealed a number of shared glycosyltransferase genes correlating to shared glycosidic linkages in the structures. One from the enzymes, encoded by only KL144 and KL86, is proposed to be a novel multifunctional rhamnosyltransfaerase likely responsible for synthesis of a shared α- l -Rha p -(1 → 2)-α-L-Rha p -(1 → 3)-L-Rha p trisaccharide fragment in the K144 and K86 structures. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01418130
- Volume :
- 193
- Database :
- Academic Search Index
- Journal :
- International Journal of Biological Macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 154048303
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2021.10.178