Influence of Mono- and Oligosaccharides on cbh1 Gene Transcription in the Filamentous Fungus Penicillium verruculosum.

A method has been developed for the analysis of the induction of cbh1 gene transcription in the filamentous fungus Penicillium verruculosum after treatment with mono- and oligosaccharides. The method makes it possible to obtain mRNA, which encodes cellobiohydrolase 1 (CBH1), in an amount sufficient for RT-qPCR. Citrate, a compound that does not cause carbon catabolite repression, was used as a carbon source. It was shown for the first time that xylose, gentiobiose, and, mainly, cellobiose induce the expression of the cbh1 gene in P. verruculosum, while sophorose and a mixture of xylooligosaccharides with a degree of polymerization of 3–5 most likely serve as precursors of inducers. [ABSTRACT FROM AUTHOR]