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Molecular diagnostics of the pigeon pea cyst nematode, Heterodera cajani Koshy, 1967, using real-time PCR.
Molecular diagnostics of the pigeon pea cyst nematode, Heterodera cajani Koshy, 1967, using real-time PCR.
- Source :
-
Nematology . 2022, Vol. 24 Issue 1, p85-90. 6p. - Publication Year :
- 2022
-
Abstract
- Summary: The pigeon pea cyst nematode, Heterodera cajani , is an important nematode pest of pigeon pea that is present in all major growing regions of this crop in India and reported from Pakistan, Egypt and Myanmar. In this study, a new real-time PCR assay for detection of H. cajani using a species-specific primer and a TaqMan probe was developed. The primers and a probe were designed to amplify the COI gene fragment. The specificity of the primer-probe set was tested in singleplex or multiplex reactions against target and non-target nematodes. In multiplex real-time PCR experiments with the specific and universal primer-probe sets, the signals were simultaneously observed for COI and D3 of 28S rRNA target genes. The results showed that the real-time PCR assay with species-specific primer and probe was sensitive enough to detect H. cajani DNA extracted from 0.003 egg or second-stage juvenile. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MOLECULAR diagnosis
*HETERODERA
*PIGEON pea
*CYSTS (Pathology)
*DNA
*RIBOSOMAL RNA
Subjects
Details
- Language :
- English
- ISSN :
- 13885545
- Volume :
- 24
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Nematology
- Publication Type :
- Academic Journal
- Accession number :
- 154336541
- Full Text :
- https://doi.org/10.1163/15685411-bja10113