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Molecular diagnostics of the pigeon pea cyst nematode, Heterodera cajani Koshy, 1967, using real-time PCR.

Molecular diagnostics of the pigeon pea cyst nematode, Heterodera cajani Koshy, 1967, using real-time PCR.

Authors :
Roubtsova, Tatiana V.
Subbotin, Sergei A.
Source :
Nematology. 2022, Vol. 24 Issue 1, p85-90. 6p.
Publication Year :
2022

Abstract

Summary: The pigeon pea cyst nematode, Heterodera cajani , is an important nematode pest of pigeon pea that is present in all major growing regions of this crop in India and reported from Pakistan, Egypt and Myanmar. In this study, a new real-time PCR assay for detection of H. cajani using a species-specific primer and a TaqMan probe was developed. The primers and a probe were designed to amplify the COI gene fragment. The specificity of the primer-probe set was tested in singleplex or multiplex reactions against target and non-target nematodes. In multiplex real-time PCR experiments with the specific and universal primer-probe sets, the signals were simultaneously observed for COI and D3 of 28S rRNA target genes. The results showed that the real-time PCR assay with species-specific primer and probe was sensitive enough to detect H. cajani DNA extracted from 0.003 egg or second-stage juvenile. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
13885545
Volume :
24
Issue :
1
Database :
Academic Search Index
Journal :
Nematology
Publication Type :
Academic Journal
Accession number :
154336541
Full Text :
https://doi.org/10.1163/15685411-bja10113