Chemiluminescence resonance energy transfer determination of uric acid with fluorescent covalent organic framework as energy acceptor.

[Display omitted] • Fluorescent TpPa-1@FL was prepared with a post-synthetic modification strategy. • TpPa-1@FL was employed an energy acceptor to construct CRET system. • The CRET signal was enhanced by bovine serum albumin. • The CRET system can determine H 2 O 2 and uric acid in biological samples. A simple and feasible strategy was developed for the preparation of fluorescent covalent organic frameworks (COFs) TpPa-1@FL. The TpPa-1–1@FL was prepared via a self-assembly strategy by soaking non-fluorescent COFs TpPa-1 into strong fluorescent fluorescein (FL) solution. A chemiluminescence resonance energy transfer (CRET) system was constructed by the combination strong fluorescent TpPa-1@FL with TCPO-hydrogen peroxide (H 2 O 2) reaction. The chemiluminescence (CL) signal of the system was further improved by the addition of bovine serum albumin (BSA). The CRET system can determine H 2 O 2 with a linear range response from 5.0 µmol/L to 20.0 mmol/L and a detection limit of 1.1 µmol/L. The CRET system was further exploited for indirect detection of uric acid with coupling of uricase. A good linear relationship was obtained for uric acid in the 10.0–400.0 µmol/L concentration range with a detection limit of 3.8 µmol/L. The practicability of this method was assessed by the determination of uric acid in real samples of human serum and urine. [ABSTRACT FROM AUTHOR]