Back to Search
Start Over
MiR-191-5p Disturbed the Angiogenesis in a Mice Model of Cerebral Infarction by Targeting Inhibition of BDNF.
- Source :
-
Neurology India . Nov/Dec2021, Vol. 69 Issue 6, p1601-1607. 7p. - Publication Year :
- 2021
-
Abstract
- <bold>Background: </bold>miRNAs are crucial regulators of angiogenesis, but there have been no detailed studies on the role of miR-191-5p in cerebral infarct angiogenesis. Here, we investigated the role of miR-191-5p in regulating cerebral infarction angiogenesis.<bold>Material and Methods: </bold>Mice were injected intracerebroventricularly with antagomir negative control (NC-antagomir), miR-191-5p antagomir, or pcDNA-BDNF 2 h before middle cerebral artery occlusion (MCAO), followed by neurobehavioral score and foot-fault test. The cerebral infarct volume was performed by TTC staining. The microvessel density was detected by FITC-dextran. RT-qPCR was used to detect the levels of miR-191-5p and its target gene BDNF. Western blotting was applied to detect the protein levels of BDNF. The luciferase reporter assay verified that miR-191-5p targeted BDNF.<bold>Results: </bold>We found an increased level of miR-191-5p in the brain tissue of mice to MCAO. Down-regulation of miR-191-5p reduced the infarct volume and ameliorated neurological deficits in MCAO mice. Further investigation showed that miR-191-5p directly targeted BDNF and that the protective effect of miR-191-5p inhibition in angiogenesis was achieved by regulating BDNF.<bold>Conclusions: </bold>Our results indicated that miR-191-5p disturbed the angiogenesis in the mouse models of cerebral infarction by inhibiting BDNF. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00283886
- Volume :
- 69
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Neurology India
- Publication Type :
- Academic Journal
- Accession number :
- 154440073
- Full Text :
- https://doi.org/10.4103/0028-3886.333459