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Structural characterization and SARS-CoV-2 inhibitory activity of a sulfated polysaccharide from Caulerpa lentillifera.
- Source :
-
Carbohydrate Polymers . Mar2022, Vol. 280, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- Caulerpa lentillifera (Bryopsidophyceae, Chlorophyta) is an edible seaweed attracting great attention for its expansion of farming scale and increasing consumption in these years. In the present study, a sulfated polysaccharide (CLSP-2) was isolated and separated from C. lentillifera , and its chemical structure was elucidated by a series of chemical and spectroscopic methods. Among these methods, mild acid hydrolysis and photocatalytic degradation were applied to release mono- and oligo-saccharide fragments which were further identified by HPLC-MSn analysis, affording the information of the sugar sequences and the sulfate substitution in CLSP-2. Results indicated that the backbone of CLSP-2 was constructed of →6)-β-Man p -(1→ with sulfated branches at C2, which were comprised of prevalent →3)-β-Gal p 4S-(1→, →3)-β-Gal p 2,4S-(1→, and minor Xyl. In addition, the virus neutralization assay revealed that CLSP-2 could effectively protect HeLa cells against SARS-CoV-2 infection with an IC 50 of 48.48 μg/mL. Hence, the present study suggests CLSP-2 as a promising agent against SARS-CoV-2. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CAULERPA
*SARS-CoV-2
*HELA cells
*MARINE algae as food
*PHOTODEGRADATION
Subjects
Details
- Language :
- English
- ISSN :
- 01448617
- Volume :
- 280
- Database :
- Academic Search Index
- Journal :
- Carbohydrate Polymers
- Publication Type :
- Academic Journal
- Accession number :
- 154594421
- Full Text :
- https://doi.org/10.1016/j.carbpol.2021.119006