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PI3K/AKT/p53 pathway inhibits infectious spleen and kidney necrosis virus infection by regulating autophagy and immune responses.

Authors :
Zhang, Xiaoting
Ming, Yue
Fu, Xiaozhe
Niu, Yinjie
Lin, Qiang
Liang, Hongru
Luo, Xia
Liu, Lihui
Li, Ningqiu
Source :
Fish & Shellfish Immunology. Jan2022, Vol. 120, p648-657. 10p.
Publication Year :
2022

Abstract

The PI3K/AKT/p53 signaling pathway is activated by various types of cellular stimuli or pathogenic infection, and then regulates fundamental cellular functions to combat these stimulations. Here, we studied the meaningful roles of PI3K/AKT/p53 in regulating cellular machine such as autophagy, immune responses, as well as antiviral activity in Chinese perch brain (CPB) cells infected by infectious spleen and kidney necrosis virus (ISKNV), which is an agent caused devastating losses in mandarin fish (Siniperca chuatsi) industry. We found that ISKNV infection induced up-regulation of host PI3K/AKT/p53 axis, but inhibited autophagy in CPB cells. Interestingly, activation of PI3K/AKT/p53 axis factors trough agonists or overexpression dramatically decreased host autophagy level, inhibited ISKNV replication, and elevated the expression of immune-related genes in CPB cells. In contrast, suppression of PI3K/AKT/p53 pathway by inhibitors or small interfering RNA (siRNA)-mediated gene silence increased the autophagy and ISKNV replication, but down-regulated immune responses in CPB cells. All these results indicate that PI3K/AKT/p53 pathway plays an important role in anti-ISKNV infection and can be used as a new target for controlling ISKNV disease. • ISKNV infection induced up-regulation of PI3K/AKT/p53 axis, but inhibited autophagy in CPB cells. • Activation of PI3K/AKT/p53 axis factors decreased autophagy level and inhibited ISKNV replication in CPB cells. • Suppression of PI3K/AKT/p53 pathway increased the autophagy and ISKNV replication in CPB cells. • The PI3K/AKT/p53 pathway promotes the expression of immune-related genes in ISKNV-infected CPB cells. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10504648
Volume :
120
Database :
Academic Search Index
Journal :
Fish & Shellfish Immunology
Publication Type :
Academic Journal
Accession number :
154619526
Full Text :
https://doi.org/10.1016/j.fsi.2021.12.046