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Paper-based immunoassay based on 96-well wax-printed paper plate combined with magnetic beads and colorimetric smartphone-assisted measure for reliable detection of SARS-CoV-2 in saliva.

Authors :
Fabiani, Laura
Mazzaracchio, Vincenzo
Moscone, Danila
Fillo, Silvia
De Santis, Riccardo
Monte, Anella
Amatore, Donatella
Lista, Florigio
Arduini, Fabiana
Source :
Biosensors & Bioelectronics. Mar2022, Vol. 200, pN.PAG-N.PAG. 1p.
Publication Year :
2022

Abstract

Coronavirus disease 2019 (COVID-19) has been recognized as a global pandemic outbreak, opening the most severe socio-economic crisis since World War II. Different scientific activities have been emerged in this global scenario, including the development of innovative analytical tools to measure nucleic acid, antibodies, and antigens in the nasopharyngeal swab, serum, and saliva for prompt identification of COVID-19 patients and to evaluate the immune response to the vaccine. The detection of SARS-CoV-2 in saliva remains a challenge for the lack of sufficient sensitivity. To address this issue, we developed a novel paper-based immunoassay using magnetic beads to support the immunological chain and 96-well wax-printed paper plate as a platform for color visualization by using a smartphone combined with Spotxel free-charge app. To assess the reliability of the measurement of SARS-CoV-2 in saliva, untreated saliva was used as a specimen and the calibration curve demonstrated a dynamic range up to 10 μg/mL, with a detection limit equal to 0.1 μg/mL. The effectiveness of this sustainable analytical tool in saliva was evaluated by comparing the data with the nasopharyngeal swab specimens sampled by the same patients and tested with Real-Time PCR reference method, founding 100% of agreement, even in the case of high Cycle Threshold (CT) numbers (low viral load). Furthermore, the positive saliva samples were characterized by the next-generation sequencing method, demonstrating the capability to detect the Delta variant, which is actually (July 2021) the most relevant variant of concern. • Colorimetric smartphone-assisted immunosensor for detection of SARS-CoV-2 in saliva. • Multiplex detection by paper-based immunosensor based on 96-well wax-printed paper. • Capability to detect the Delta variant, positive samples characterized by the NGS method. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09565663
Volume :
200
Database :
Academic Search Index
Journal :
Biosensors & Bioelectronics
Publication Type :
Academic Journal
Accession number :
154660336
Full Text :
https://doi.org/10.1016/j.bios.2021.113909