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Expression of functional interleukin-21 receptor on adult T-cell leukaemia cells.
- Source :
-
British Journal of Haematology . Jan2005, Vol. 128 Issue 2, p169-176. 8p. - Publication Year :
- 2005
-
Abstract
- Adult T-cell leukaemia (ATL) is caused by human T-cell leukaemia virus type I (HTLV-I). It has been suggested that cytokines play a role in the development and in the neoplastic cell growth of ATL. However, the precise mechanism involved in this process still remains unclear. Interleukin-21 (IL-21) and its receptor (IL-21R) have been recently described. In this study, we examined the expression of IL-21R and the effect of IL-21 on ATL cells. Real-time reverse transcription polymerase chain reaction showed that HTLV-I-infected cell lines and primary ATL cells expressed IL-21R mRNA. Cell surface expression of IL-21R on these cells was confirmed by flow cytometric analysis using a newly developed monoclonal antibody against human IL-21R. In contrast to the expression of IL-21R, IL-21 mRNA was scarcely detectable in these cells. Notably, IL-21 induced the proliferation of ATL-43T and ED-40515(+) cells, both of which were derived from leukaemic cell clones of ATL. Concerning the intracellular signalling pathways, IL-21 activated the phosphorylation of the signal transducers and activators of transcription (STAT)3 and STAT5. Taken together, these findings provide the first evidence that ATL cells express functional IL-21R, suggesting that it may contribute to the pathophysiology of ATL. In addition, the IL-21/IL-21R system may represent a new target for the treatment of ATL. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ADULT T-cell leukemia
*CYTOKINES
*HTLV-I
*CELLULAR immunity
*ANEMIA
*HTLV diseases
Subjects
Details
- Language :
- English
- ISSN :
- 00071048
- Volume :
- 128
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- British Journal of Haematology
- Publication Type :
- Academic Journal
- Accession number :
- 15477466
- Full Text :
- https://doi.org/10.1111/j.1365-2141.2004.05255.x