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Colorimetric logic gate for protamine and trypsin based on the Bpy-Cu nanozyme with laccase-like activity.
- Source :
-
Sensors & Actuators B: Chemical . Apr2022, Vol. 357, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- A novel logic gate for protamine and trypsin was developed based on a nanozyme with laccase-like activity. It is found for the first time that the nanomaterial (Bpy-Cu) made from 4,4′-bipyridine (Bpy) and Cu2+ has significant laccase-like activity, which could oxidize the typical laccase substrate (2,4-dichlorophenol) and the oxidation product was coupled with 4-aminoantipyrine to form a red dye product. It is also observed that heparin (Hep) can bond to the surface of Bpy-Cu, and obviously enhanced the laccase-like activity of Bpy-Cu by showing a stronger colorimetric reaction. In addition, because of the strong interaction between heparin and protamine, the addition of protamine to the complex of Bpy-Cu/Hep caused the decrease of surface charges as well as the laccase-like activity. Furthermore, protamine could be hydrolyzed by trypsin, and the inhibited laccase-like activity of Bpy-Cu/Hep complex by protamine could be recovered by trypsin. According to the above results, a novel sensing strategy for protamine and trypsin, and an IMPLICATION logic gate was developed. This work provided a new sensing pathway for biological molecules on the basis of the controlled regulation the laccase-like activity of nanozymes. • Laccase-like activity of Bpy-Cu was first investigated. • Heparin enhanced laccase-like activity of Bpy-Cu was observed. • Protamine and trypsin were detected with satisfactory results. • An "IMPLICATION" logic gate based on Bpy-Cu was constructed successfully. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 357
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 155090512
- Full Text :
- https://doi.org/10.1016/j.snb.2022.131429