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Developmental validation of the Microreader™ RM-Y ID System: a new rapidly mutating Y-STR 17-plex system for forensic application.
- Source :
-
International Journal of Legal Medicine . Mar2022, Vol. 136 Issue 2, p501-512. 12p. - Publication Year :
- 2022
-
Abstract
- Y-chromosomal short tandem repeats (Y-STRs) are widely applied to evolutionary, genealogical, and kinship analyses of male linages in forensic studies, but these low to midrange mutated Y-STRs typically fail to separate related males from the same paternal lineage. Recently, rapidly mutating Y-STRs (RM Y-STRs) have been demonstrated to improve the differentiation of male relatives and individuals. The Microreader™ RM-Y ID System is a new RM Y-STR kit that is capable of simultaneously amplifying 17 RM Y-STRs. Herein, to verify the efficiency and accuracy of the Microreader™ RM-Y ID System, developmental validation was conducted, including PCR-based studies, sensitivity, stability, species specificity, mixture, stutter percentage, and precision studies. Full profiles could be obtained when the hematin concentration was 250 μM, humic acid concentration was 1500 ng/μl, and tannic acid concentration was 200 ng/μl. Full profiles of the mixture of males/males could be detected up to a ratio of 19:1, and full profiles of females/males could always be detected even at ratios up to 24,000:1. Moreover, the forensic characteristics of 250 DNA-confirmed father-son pairs were analysed. The results showed that these 17 RM Y-STRs had high power for forensic discrimination (HD = 1) in the Chinese Han population, and the mutation rates were in the range of 4 × 10−3 (95% CI 1.00 × 10−4 to 2.21 × 10−2, DYS464) to 8.8 × 10−2 (95% CI 5.60 × 10−2 to 1.30 × 10−1, DYF399S1), indicating that the kit was effective for RM Y-STR studies and absolute individualisation of interrelated male individuals. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HUMIC acid
*TANNINS
*CHINESE people
*SPECIES specificity
*HEME
Subjects
Details
- Language :
- English
- ISSN :
- 09379827
- Volume :
- 136
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- International Journal of Legal Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 155262589
- Full Text :
- https://doi.org/10.1007/s00414-021-02632-w