Immunomodulatory Effect of Isolated Triterpenes from Alstonia Scholaris Linn. In Wistar Albino Rats.

Aim: Immunomodulatory Effect of Isolated Triterpenes from Alstonia scholaris Linn. in Wistar Albino Rats. Material & Methods: The flower part of Alstonia scholaris were extracted by using n-hexane, dichloromethane, methanol, and water. All the extracts were screened for the presence of various active compounds. DCM extract showed the occurrence of terpenoids and flavonoids. The methanolic extract showed the presence of flavonoids and other active components. DCM extract was then allowed to isolate the active compound by the means of column chromatography. N-hexane and dichloromethane solvents in various ratios were used for the isolation of active compounds. Then isolated compound was identified by the various spectral analysis techniques. The isolated compound was evaluated by cyclophosphamide and E. Coli model. Results: In the case of cyclophosphamide induced myelosuppression, there was a decrease in the WBC reckoning in the control group. In treatment groups, the WBC count was found to be augmented with p<0 .01and p< 0.001 at 10 and 50 mg/kg dose respectively on the 11th day. In E. coli induced abdominal sepsis, mortality due to peritonitis was evaluated in control and treatment groups. There was 100% mortality in the control group. In the groups treated with a-amyrin 10 mg/kg body, wt 36 % mortality was observed respectively. Conclusion: The current discoveries are huge for the improvement of options, cheap and maybe safer strategies for the treatment of diseases. A detailed study is also required on structure determination of the compounds from bioactive fractions in order to find the structure- activity relationship. [ABSTRACT FROM AUTHOR]