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Optimization of sensing-pad functionalizing strategy toward separative extended-gate FET biosensors for PSA detection.
- Source :
-
Journal of Pharmaceutical & Biomedical Analysis . Mar2022, Vol. 211, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- • The stability and sensitivity of FET biosensors are improved via decorating the Au sensing pads with T-ZnO. • T-ZnO helps construct a 3D and concave detection front with many biological modification sites and small Debye volume. • T-ZnO helps improve long-term air stability/test repeatability in bio-solution by releasing the stress and erosion effect. • Biosensors with a chemical bio-functionalized T-ZnO front show a wide detection scope, good sensitivity and stability. [Display omitted] In this study, separative extended-gate AlGaAs/GaAs high electron mobility transistor (HEMT) biosensor is proposed for prostate-specific antigen (PSA) detection. Zinc oxide nanotetrapods (T-ZnO) with a four-leg structure is introduced onto the sensing pad to form a three-dimensional (3D) and concave detection front. Compared with common plane front, the elaborately-designed-3D and concave detection front can offer more biological modification sites and decrease the Debye volume, resultantly improving both the detection scope and sensitivity. Anti-PSA probes can be fixed at any sites of T-ZnO via a chemical bio-functionalization method, which facilitates better detection performance by comparison with a physical modification scheme. On the other hand, the T-ZnO nanostructures with the four-leg configuration are capable of releasing the stress and erosion effect of the solution on the plane Au film, contributing to a great improvement in the reliability of the biosensors. The optimized biosensors with chemical bio-functionalized T-ZnO detection front demonstrate good linear current/voltage response to label-free PSA target in the concentration range from 5 fg/ml~5 ng/ml and a sensitivity variation ~ 1.3% dec−1. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MODULATION-doped field-effect transistors
*BIOSENSORS
*PROSTATE-specific antigen
Subjects
Details
- Language :
- English
- ISSN :
- 07317085
- Volume :
- 211
- Database :
- Academic Search Index
- Journal :
- Journal of Pharmaceutical & Biomedical Analysis
- Publication Type :
- Academic Journal
- Accession number :
- 155629149
- Full Text :
- https://doi.org/10.1016/j.jpba.2022.114597