Molecularly imprinted polymer-based electrochemical impedimetric sensors on screen-printed carbon electrodes for the detection of trace cytokine IL-1β.

In this study, protein-imprinted sensors were electrochemically fabricated on screen-printed carbon electrodes (SPCEs) for the cytokine interleukin-1β (IL-1β) detection. A double layer comprising poly(o -phenylenediamine) and poly(chromotrope 2R) with a template (i.e., IL-1β biomacromolecules) was formed through the cyclic voltammetry (CV) technique to modify the molecularly imprinted polymer (MIP) films on the SPCEs. The electrochemical sensing properties were investigated via CV and electrochemical impedance spectroscopy to confirm the imprinting effect on the MIP films. The results show that the MIP sensor has a highly sensitive response in the trace IL-1β solution (a few pg/mL) with a limit of detection of 0.23 pg/mL and a limit of quantification of 0.78 pg/mL. Furthermore, the MIP sensor showed high selectivity for IL-1β adsorption compared to other proteins. In summary, based on binary double layers, the impedance sensing platforms of electropolymerized MIP films show potential application in the practical detection of macromolecular proteins. [Display omitted] ● Cytokine interleukin-1β differential pulse voltammetric and impedimetric chemosensors. ● Electropolymerized chromotrope 2R for protein imprinting. ● A thin bulk imprinted films were optimized by controlling film thickness. ● Electrochemical impedimetric sensing responses on molecularly imprinted polymers. ● High sensitivity and selectivity in determining trace interleukin-1β. [ABSTRACT FROM AUTHOR]