Separation and detection of E. coli O157:H7 using a SERS-based microfluidic immunosensor.

Keywords: Food-borne pathogen; Raman spectroscopy; Immunoassay; Food safety; Gold nanoparticles EN Food-borne pathogen Raman spectroscopy Immunoassay Food safety Gold nanoparticles 1 10 10 03/23/22 20220301 NES 220301 Supplementary Information The online version contains supplementary material available at https://doi.org/10.1007/s00604-022-05187-8. SERS nanoprobes, containing gold nanoparticles and specific antibodies against I E. coli i O157:H7, were used to selectively anchor onto the I E. coli i O157:H7 cells and separate them from lettuce samples. It is worth noting that the used SERS nanoprobes are selective for I E. coli i O157:H7 in the presence of other pathogens but do not act selectively for I E. coli i O157:H7 in the presence of I E. coli i K12 strains. [Extracted from the article]