Back to Search Start Over

MiR-106b-5p regulates esophageal squamous cell carcinoma progression by binding to HPGD.

Authors :
Yang, Fan
Sun, Zhanwen
Wang, Dengyun
Du, Tian
Source :
BMC Cancer. 3/22/2022, Vol. 22 Issue 1, p1-17. 17p.
Publication Year :
2022

Abstract

<bold>Background: </bold>Several studies have documented the key role of microRNAs (miRNAs) in esophageal squamous cell carcinoma (ESCC). Although the expression of the 15-hydroxyprostaglandin dehydrogenase (HPGD) gene and miR-106b-5p are reportedly linked to cancer progression, their underlying mechanisms in ESCC remain unclear.<bold>Methods: </bold>mRNA and miRNA expression in ESCC tissues and cells were analyzed using RT-qPCR. Luciferase and RNA pull-down assays were used to identify the interaction between miR-106b-5p and HPGD. Xenograft and pulmonary metastasis models were used to assess tumor growth and metastasis. CCK-8, BrdU, colony formation, adhesion, cell wound healing, Transwell, and caspase-3/7 activity assays, and flow cytometry and western blot analyses were used to examine the function of miR-106-5p and HPGD in ESCC cell lines.<bold>Results: </bold>The findings revealed that miR-106b-5p expression was upregulated in ESCC tissues and cell lines. miR-106b-5p augmented cellular proliferation, colony formation, adhesion, migration, invasion, and proportion of cells in the S-phase, but reduced apoptosis and the proportion of cells in G1-phase. Silencing of miR-106-5p inhibited tumor growth in vivo and pulmonary metastasis. Although HPGD overexpression suppressed proliferation, colony formation, adhesion, migration, and invasion of ESCC cells, it promoted apoptosis and caused cell cycle arrest of the ESCC cells. The results also indicated a direct interaction of HPGD with miR-106b-5p in ESCC cells. Furthermore, miR-106b-5p inhibited HPGD expression, thereby suppressing ESCC tumorigenesis.<bold>Conclusion: </bold>Our data suggest that miR-106b-5p enhances proliferation, colony formation, adhesion, migration, and invasion, and induces the cycle progression, but represses apoptosis of ESCC cells by targeting HPGD. This suggests that the miR-106b-5p/HPGD axis may serve as a promising target for the diagnosis and treatment of ESCC. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14712407
Volume :
22
Issue :
1
Database :
Academic Search Index
Journal :
BMC Cancer
Publication Type :
Academic Journal
Accession number :
155909167
Full Text :
https://doi.org/10.1186/s12885-022-09404-8