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Preparation and characterization of bovine serum albumin nanoparticles modified by Poly-l-lysine functionalized graphene oxide for BMP-2 delivery.
- Source :
-
Materials & Design . Mar2022, Vol. 215, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- [Display omitted] • Our study developed Go-PLL complexes as novel modification nanomaterials for BMP-2 delivery. • GO-PLL/BMP-2 nanoparticles controlled BMP-2 release velocity and prolong release period. • GO-PLL/BMP-2 nanoparticles significantly promoted osteogenic differentiation of BMSCs and exhibited favorable bone formation ability. The development of bioactive carrier with controlled delivery of Bone morphogenetic protein (BMP-2) is an important part in bone regeneration. Our study fabricated novel modified nanomaterials based on poly-l-lysine functionalized graphene oxide (GO-PLL) and explore its potential applications for BMP-2 delivery. GO-PLL was used to modify BMP-2 encapsulated bovine serum albumin (BSA) nanoparticles (GO-PLL/BMP-2) by self-assembling technology. The physiochemical and biological properties of GO-PLL modified BSA nanoparticles were investigated in vitro. Additionally, rabbit calvarial bone defects were created to investigate bone formation ability in the presence of GO-PLL/BMP-2 loaded alginate/gelatin scaffolds. The results suggested BSA particles were spatially interacted with GO-PLL complexes by strong electrostatic attraction. When compared with BMP-2 encapsulated BSA nanoparticles, a pronounced decrease in BMP-2 gentle release was observed in the presence of GO-PLL/BMP-2 nanoparticles, and extended to over 14-day release periods. More importantly, GO-PLL/BMP-2 nanoparticles significantly enhanced alkaline phosphatase activity (ALP) and matrix mineralization, and exhibited favorable bone formation ability when loaded into alginate/gelatin scaffolds. These results demonstrated that PLL functionalized GO complexes are considered as appropriate delivery system for BSA modification, and have great potential for BMP-2 delivery in bone regeneration. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02641275
- Volume :
- 215
- Database :
- Academic Search Index
- Journal :
- Materials & Design
- Publication Type :
- Academic Journal
- Accession number :
- 155962849
- Full Text :
- https://doi.org/10.1016/j.matdes.2022.110479