Impact of celluloses and pectins restrictions on gluten development and water distribution in potato-wheat flour dough.

The addition of potato to wheat flour extends the nutritional values of bread. However, the adverse effects mediated by high dietary fiber in potato flour could affect the formation of gluten matrix. The water dynamics and distribution determined by the Low field nuclear magnetic resonance (LF-NMR) demonstrated a competitive water binding of dietary fiber, resulting in the partial dehydration and conformational changes of gluten protein complexes. Besides, the microstructure of the dough characterized by Scanning electron microscope (SEM) suggested that the insoluble cellulose could block the continuity of gluten from the spatial position, thereby negative affecting the mechanical properties of the dough. In our study, addition of cellulase and/or pectinase apparently mitigated the gluten aggregation and dehydration, contributing to the formation and the continuity of the three-dimensional gluten network. As a consequence, the specific volume of the bread was increased by 40.2%, and the hardness was reduced by 64.48%. • Potato flour leads to the aggregation or abnormal folding of protein complexes. • Competitive water binding of dietary fiber induces partial dehydration of gluten. • The steric hindrance of cellulose impedes the spread of gluten matrix. • Enzymatic treatment makes the potato-wheat flour dough more flexible. [ABSTRACT FROM AUTHOR]