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Turn-on fluorescent inner filter effect-based B,S,N co-doped carbon quantum dots and vanadium oxide nanoribbons for α-glucosidase activity detection.
- Source :
-
Microchemical Journal . Jul2022, Vol. 178, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- [Display omitted] • Hybrid materials of BSN-CQDs and vanadium oxide nanoribbons have been prepared. • A method for detection of α-glucosidase has been established. • The "turn-on" and "turn-off" processes have been obtained. • The satisfactory monitor results have been obtained. A sensitive and effective strategy for the screening of α-glucosidase and its inhibitors was established based on carbon quantum dots (CQDs) and vanadium oxide nanoribbons. B,S,N co-doped CQDs (BSN-CQDs) were prepared by one-step microwave hydrothermal method using o-phenylenediamine, boric acid, and thiourea as raw materials. The prepared CQDs emitted yellow fluorescence (λ em = 565 nm) under excitation of 410 nm, and the quantum yield was 15.3%. The fluorescence of CQDs was firstly quenched by the vanadium oxide nanoribbons via inner filter effect. Then, 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA2G) was introduced as a substrate of α-glucosidase to produce ascorbic acid (AA). Vanadium oxide nanoribbons could be converted to V4+ by AA, which led to fluorescence recovery. After adding inhibitors (anti-diabetic drugs), the α-glucosidase activity was inhibited and the degree of fluorescence recovery was reduced. The established nanoprobe showed a response linearity to α-glucosidase in the range of 0.01–5 U mL−1, with a low detection limit of 0.003 U mL−1. This platform was applied for the screening of acarbose, with a detection limit of 0.02 μM. The constructed sensing strategy had the advantages of high selectivity, sensitivity, and was successfully used for α-glucosidase detection in serum samples and anti-diabetic drugs screening. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0026265X
- Volume :
- 178
- Database :
- Academic Search Index
- Journal :
- Microchemical Journal
- Publication Type :
- Academic Journal
- Accession number :
- 156228133
- Full Text :
- https://doi.org/10.1016/j.microc.2022.107405