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Enzymatic biosensor for nitrite detection based on direct electron transfer by CPO-ILEMB/Au@MoS2/GC.
- Source :
-
Journal of Applied Electrochemistry . Jun2022, Vol. 52 Issue 6, p979-987. 9p. - Publication Year :
- 2022
-
Abstract
- A novel nitrite electrochemical biosensor is constructed by glassy carbon electrodes (GC) coated by chloroperoxidase (CPO) -Au nanoparticles-MoS2 nanoflowers in the presence of ionic liquid, 1-ethyl-3-methylimidazolium bromide (EMB-IL). Au-MoS2 nanoflowers modified nanocomposites are synthesized using sodium carboxymethyl cellulose as reducing agent and stabilizer. The EMB-IL can effectively promote direct electron transfer between CPO and electrode surface without redox mediator. Herein, the surface concentration of CPO on the electrode surface is 1.36 × 10–10 mol cm−2, and the electron transfer coefficient and the rate constant of CPO at electrode surface are 0.87 and 2.03 s−1, respectively. The proposed nitrite electrochemical biosensor shows a wide linear range, low detection limit, and high sensitivity. The biosensor was applied to the determination of nitrite in pickled cabbage with a good accuracy and recovery. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0021891X
- Volume :
- 52
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Journal of Applied Electrochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 156415169
- Full Text :
- https://doi.org/10.1007/s10800-022-01689-8